lachnospiraceae family member k alysoides  (ATCC)

Journal: The Journal of Experimental Medicine

Article Title: Suppression of local type I interferon by gut microbiota–derived butyrate impairs antitumor effects of ionizing radiation

doi: 10.1084/jem.20201915

Figure Lengend Snippet: Vancomycin decreased Lachnospiraceae in the gastrointestinal tract, while both Lachnospiraceae supplementation and butyrate administration impaired the antitumor effect of IR, in a melanoma model. (A) Verification of the abundance of Lachnospiraceae by qPCR using Lachnospiraceae (TSD-26) genomic DNA as a standard ( n = 4/group). Unpaired t tests were used to analyze the data. (B) GF C57BL/6 mice ( n = 5/group) were fed Lachnospiraceae and K. alysoides (TSD-26) for 1 mo, 10 8 CFU/mouse weekly. The control group was fed the same volume of autoclaved water. Then the mice were injected s.c. with 10 6 B16F1 cells, and established tumors were irradiated (20 Gy) on day 10 after tumor inoculation. (C) C57BL/6 mice ( n = 5/group) were injected s.c. with 10 6 B16F1 cells. Established tumors were irradiated (20 Gy) on day 10 after tumor inoculation. Tumor-bearing mice were injected i.v. with 4 μmol of NaBu on days 1, 4, and 7 after IR. (D) C57BL/6 mice ( n = 5/group) were injected s.c. with 10 6 B16F1 cells. Established tumors were irradiated (20 Gy) on day 10 after tumor inoculation. Tumor-bearing mice were intratumorally (i.t.) injected with 2 μmol of NaBu on days 1, 4, and 7 after IR. A tumor growth curve is shown. Two-way ANOVA tests were used to analyze the tumor growth data. *, P < 0.05; ****, P < 0.0001. One representative experiment (out of two experiments) is shown.

Article Snippet: After gavage, K. alysoides (TSD-26; American Type Culture Collection [ATCC])–colonized mice had a decreased antitumor response to IR compared with control mice in both MC38 and B16F1 tumor models ( ).

Techniques: Control, Injection, Irradiation

Journal: The Journal of Experimental Medicine

Article Title: Suppression of local type I interferon by gut microbiota–derived butyrate impairs antitumor effects of ionizing radiation

doi: 10.1084/jem.20201915

Figure Lengend Snippet: Lachnospiraceae and butyrate restrict the antitumor effect of IR. (A) GF C57BL/6 mice were fed with Lachnospiraceae and K. alysoides (TSD-26) for 1 mo, 10 8 CFU/mouse weekly. The control group was fed the same volume of autoclaved water. Mice were injected s.c. with 10 6 MC38 cells, and established tumors were irradiated (20 Gy) on day 10 after tumor inoculation. On day 10 after tumor inoculation, sera were collected for butyrate detection by GC/MS. A tumor growth curve is shown ( n = 5/group). (B) GF C57BL/6 mice were fed HI TSD-26 weekly for 1 mo. 10 8 CFU of TSD-26 were HI at 100°C for 30 min and fed to one mouse. The control group was fed the same volume of autoclaved water. Then the mice were injected s.c. with 10 6 MC38 cells, and established tumors were irradiated (20 Gy) on day 10 after tumor inoculation. A tumor growth curve is shown ( n = 5/group). On day 10 after tumor inoculation, sera were collected for butyrate detection by GC/MS. (C) Butyrate concentration in sera collected from A and B (control group, n = 10; TSD-26 group, n = 10; HI TSD-26 group, n = 12). Data were pooled from two independent experiments. (D) C57BL/6 mice were injected s.c. with 10 6 MC38 cells. Established tumors were irradiated (20 Gy) on day 10 after tumor inoculation. Tumor-bearing mice were injected i.v. with 4 μmol of NaBu on days 1, 4, and 7 after IR. A tumor growth curve is shown ( n = 5/group). Two-way ANOVA tests were used to analyze the tumor growth data, and unpaired t tests were used to analyze the other data. n.s., not significant; *, P < 0.05; **, P < 0.01; ****, P < 0.0001. One representative experiment (out of two experiments in A and B or three experiments in D) is shown.

Article Snippet: After gavage, K. alysoides (TSD-26; American Type Culture Collection [ATCC])–colonized mice had a decreased antitumor response to IR compared with control mice in both MC38 and B16F1 tumor models ( ).

Techniques: Control, Injection, Irradiation, Gas Chromatography-Mass Spectrometry, Concentration Assay

Journal: The Journal of Experimental Medicine

Article Title: Suppression of local type I interferon by gut microbiota–derived butyrate impairs antitumor effects of ionizing radiation

doi: 10.1084/jem.20201915

Figure Lengend Snippet: HI Lachnospiraceae species do not affect intratumoral butyrate concentration. GF C57BL/6 mice ( n = 5/group) were fed HI Lachnospiraceae and K. alysoides (TSD-26) weekly for 1 mo. 10 8 CFU of TSD-26 were HI at 100°C for 30 min and fed to one mouse. The control group was fed the same volume of autoclaved water. Then the mice were injected s.c. with 10 6 MC38 cells. On day 10 after tumor inoculation, tumors (control group, n = 14; HI-TSD-26 group, n = 10) were collected for butyrate detection by GC/MS. Unpaired t tests were used to analyze the data. n.s., not significant. Data were pooled based on two experiments yielding similar results.

Article Snippet: After gavage, K. alysoides (TSD-26; American Type Culture Collection [ATCC])–colonized mice had a decreased antitumor response to IR compared with control mice in both MC38 and B16F1 tumor models ( ).

Techniques: Concentration Assay, Control, Injection, Gas Chromatography-Mass Spectrometry